Modulation of gastric H+ , K+ -transporting ATPase function by sodium
نویسندگان
چکیده
منابع مشابه
K+)-transporting ATPase system
The distribution of free thiol groups associated with the membrane proteins of the purified pig gastric microsomal vesicles was quantified, and the relation of thiol groups to the function of the gastric (H+ + K+)-transporting ATPase system was investigated. Two different thiol-specific agents, carboxypyridine disulphide (CPDS) and N(1-naphthyl)maleimide (NNM) were used for the study. The struc...
متن کاملCharacterization of a beta subunit of the gastric H+/K(+)-transporting ATPase.
The catalytic subunit of the H+/K(+)-transporting ATPase (EC 3.6.1.3) has 62% identity to the alpha, or catalytic subunit, of the Na+/K(+)-transporting ATPase (EC 3.6.1.37); however, a homologous beta subunit was unknown until recently. Removal of the carbohydrate from purified hog H+/K(+)ATPase vesicles reveals a 35-kDa peptide that, when fragmented with protease V8, gives sequences homologous...
متن کاملInhibition of the gastric H,K-ATPase by clotrimazole.
The antimycotic drug clotrimazole inhibits the function of the gastric H,K-ATPase in a manner similar to that observed for the Na,K-ATPase. Because of the high hydrophobicity of the compound, the interaction between clotrimazole and the ion pump occurs at the membrane domain in the apolar core of the membrane. The enzymatic activity was inhibited with a half-saturating concentration of 5.2 micr...
متن کاملRegulation of the gastric microsomal (H+ + K+)-transporting ATPase system by the endogenous activator. Effect of phospholipase A2 treatment.
Pig gastric microsomal (H+ + K+)-stimulated ATPase activity was nearly abolished within 10 min of digestion with phospholipase A2 at room temperature. The enzyme activity could be largely restored by a cytosolic activator protein partially purified from the gastric cells. The K+ sensitivity and turnover of 32P-labelled intermediates produced by the control and the activator-reconstituted micros...
متن کاملCation selectivity of gastric H,K-ATPase and Na,K-ATPase chimeras.
Chimeras of the catalytic subunits of the gastric H,K-ATPase and Na, K-ATPase were constructed and expressed in LLC-PK1 cells. The chimeras included the following: (i) a control, H85N (the first 85 residues comprising the cytoplasmic N terminus of Na,K-ATPase replaced by the analogous region of H,K-ATPase); (ii) H85N/H356-519N (the N-terminal half of the cytoplasmic M4-M5 loop also replaced); a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1985
ISSN: 0014-5793
DOI: 10.1016/0014-5793(85)80733-x